Triiodothyronine (T3)

Triiodothyronine, also known as T3, is a thyroid hormone. It affects almost every physiological process in the body, including growth and development, metabolism, body temperature, and heart rate.

The determination of triiodothyronine is one of the sensitive indexes in the diagnosis of hyperthyroidism.
When elevating hyperthyroidism, it is also the precursor of the recurrence of hyperthyroidism. In addition, pregnancy and acute hepatitis will advance Hypothyroidism, simple goiter, acute and chronic nephritis, chronic hepatitis, cirrhosis of the liver.
T3 can be used to diagnose t3 antibodies, early detection of hyperthyroidism, and diagnosis of pseudo thyrotoxicosis.

The serum T3 and T4 levels changes are generally consistent, which is a sensitive indicator of thyroid function diagnosis, especially important for early diagnosis. It is a specific diagnostic indicator of T3 hyperthyroidism.                                                                            
Types Of Triiodothyronine (T3)
Antibody Application
Sheep Anti-human T3 mAb For immunodiagnostic: ELISA, LFA, CLIA
Triiodothyronine Intro
As the largest endocrine gland in the human body, the thyroid secretes the main active substances, tetraiodothyronine (T4) and triiodothyronine (T3), which are extremely important for protein synthesis thermoregulation, energy production, and regulation effect.

T3 plays a vital role in judging the physiological condition of the human body, so it is of great significance to detect the content of T3 in serum. The content of T3 in serum is 1/80~1/50 of that of T4, but the biological activity of T3 is 5~10 times that of T4. Most of the T3 in serum is converted from the deiodination of peripheral tissues. A small part of T3 is directly secreted by the thyroid and released into the blood. Most of the T3 in serum is bound to binding proteins. About 90% is bound to thyroxine-binding globulin (TBG), the rest is bound to albumin, and a tiny amount is attached to thyroxine-binding prealbumin (TBPA).
Free T3 Test
Increased T3 is seen in hyperthyroidism, early stage of triiodothyronine-type hyperthyroidism crisis, iodine-deficiency goiter, and hyperthyroid-binding globulinemia.
It is decreased in hypothyroidism, low thyroxine-binding globulinemia, and so on.

High result possible disease: hyperthyroidism.

Low result possible diseases: Hypothyroidism, endocrine proptosis, thyroiditis, resting lymphocytic thyroiditis, hyperthyroidism in children, hyperthyroidism in the elderly, thyroid tuberculosis, endocrine disorders, euthyroid goiter, precocious children Breast hypertrophy.
TT3 Performance Data
Manufacturer SEKBIO
Parameter TT3
Linearity 0.3-10 nmol/L
Detection limit 0.3 nmol/L
Benchmark product Roche
Test principle and procedure TT3: Add 30 μl of sample + 30 μl of Rd, incubation for 10 minutes without cleaning, 30 μl of Ra + 30 μl of Rc and incubation for 5 minutes. Wash with 200 μl of washing solution. Finally, add 100 μl of A + 100 μl of B.

Precision
Sample RLU Concentration Sample RLU Concentration





0.5 270,946 0.47




5 83,336 4.10
276,899 0.45 79,406 4.42
278,536 0.44 82,669 4.16
259,590 0.52 84,427 4.02
269,690 0.48 81,363 4.26
270,230 0.47 79,263 4.44
257,837 0.53 80,565 4.33
270,880 0.47 77,389 4.60
279,936 0.44 81,233 4.27
271,277 0.47 79,113 4.45
AVE 270582.10 0.47 AVE 80876.40 4.30
SD 7269.04 0.03 SD 2169.67 0.18
CV 2.7% 6.1% CV 2.7% 4.2%

Limit of detection
Sample RLU Concentration




Ag Diluent 980,305 1,022,449 0 0
986,963 1,001,873 0 0
984,209 1,003,978 0 0
1,026,252 1,000,042 0 0
1,018,574 961,333 0 0
974,731 982,278 0 0
1,022,323 969,066 0 0
1,071,730 968,489 0 0
1,043,724 1,028,076 0 0
1,014,595 1,014,797 0 0
AVE 1003789.35 0.00
SD 28331.30 0.00
AVE+2*SD 0

Linear
Sample（nmol/L) Dilution ratio Concentration value Average
10.13 1.0 10.41 10.67 10.13 10.41
8.14 0.8 8.02 7.86 8.03 7.97
6.15 0.6 5.96 5.90 5.94 5.94
4.16 0.4 3.67 3.72 3.66 3.68
2.16 0.2 1.79 1.75 1.70 1.75
0.17 0.0 0.17 0.18 0.16 0.17


Method Comparison

Reagents Stability
Reagent Sample(nmol/L) Average Deviation
2-8℃ 9.18 55,377
4.71 81,863
0.43 340,779
Antigen diluent 666,737
random sample 200,281
calf serum 453,520
37℃ 1d 9.18 53,767 -2.91%
4.71 83,258 1.70%
0.43 339,943 -0.25%
Antigen diluent 664,104 -0.39%
random sample 188,472 -5.90%
calf serum 459,529 1.32%
37℃ 3d 9.18 51,653 -6.72%
4.71 82,714 1.04%
0.43 360,888 5.90%
Antigen diluent 645,376 -3.20%
random sample 198,372 -0.95%
calf serum 480,304 5.91%
37℃ 7d 9.18 49,977 -9.75%
4.71 78,711 -3.85%
0.43 366,369 7.51%
Antigen diluent 629,915 -5.52%
random sample 189,952 -5.16%
calf serum 486,154 7.20%

Calibrator Stability
Reagent Sample(nmol/L) Average Deviation
2-8℃ 6.70 43,902
3.70 74,473
0.32 310,740
37℃ 1d 6.70 43,667
3.70 76,993 -0.53%
0.32 307,831 3.38%
37℃ 3d 6.70 43,303 -0.94%
3.70 75,963 -1.36%
0.32 306,706 2.00%

37℃ 7d
6.70 44,214 -1.30%
3.70 72,454 0.71%
0.32 305,293 -2.71%

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