Rat Calprotectin(Calprotectin) ELISA Kit

Product name

Rat Calprotectin(Calprotectin) ELISA Kit

Catalog NO.

FY-ER4603

Reactivity

Rat

Assay range

1.56-100ng/mL

Sensitivity

0.57ng/mL

Detection Method

Double-antibody Sandwich

Size

48T, 96T

Storage

2-8 ℃ for 6 months

CV %（）

Intra-Assay: CV<10%
Inter-Assay: CV<12%

Note

For Research Use Only

Calprotectin(Calprotectin) Basic information

Calprotectin is a complex of the mammalian proteins S100A8 and S100A9.Other names for calprotectin include MRP8-MRP14, calgranulin A and B, cystic fibrosis antigen, L1, 60BB antigen, and 27E10 antigen. In the presence of calcium, calprotectin is capable of sequestering the transition metals iron, manganese and zinc via chelation.This metal sequestration affords the complex antimicrobial properties.Calprotectin is the only known antimicrobial manganese sequestration protein complex.Calprotectin comprises as much as 60% of the soluble protein content of the cytosol of a neutrophil, and it is secreted by an unknown mechanism during inflammation.Faecal calprotectin has been used to detect intestinal inflammation (colitis or enteritis) and can serve as a biomarker for inflammatory bowel diseases. Blood based calprotectin (in serum and plasma) is used in diagnostics of multiple inflammatory diseases, including autoimmune diseases, like arthritis, and severe infections including sepsis.

Rat Calprotectin(Calprotectin) ELISA Kit test method

Feiyue’s Rat Calprotectin Elisa kit is an ELISA reagent for detection of Neutrophil elastase in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

This kit uses sandwich ELISA to detect the concentration of Rat Calprotectin.Rat Calprotectin -specific monoclonal antibody has been pre-coated in the wells of the supplied microplate. Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti- Rat Calprotectin antibody with HRP-Streptavidin. TMB solution is added to react with the sandwich for ming optical signal measured by microplate reader. The concentration of Rat Calprotectin in the sample can be calculated by comparing the absorbance of the sample with the standard curve.