Human Collagen, Type I, Alpha 1 ELISA kit

NE01C1899 Human Collagen, Type I, collagen 1 elisa kit

Human Collagen, type I, collagen type i alpha 1 kit is suitable for the detection of samples from human species. Collagen, type I, Alpha 1 can also be called collagen type I alpha 1, collagen type I alpha 1 chain, EDSARTH1, COL1A1, EDSC, OI1, OI2, OI3, OI4, CAFYD.

Principle of the Assay

COL1A1 ELISA kit is applied to the solid phase sandwich Enzyme Linked Immunosorbent Assay. The microtiter plate has been pre-coated with capture antibody. The antigen to be measured and the biotin-conjugated detection antibody are then added to the microtiter plate wells sequentially to form a capture antibody-antigen-detection antibody complex. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells, and then a TMB substrate solution is added to the wells to incubate. TMB substrate solution turns blue after the oxidation of HRP, and finally turns to yellow immediately after adding the stop solution. Color of TMB substrate positively correlated with total COL1A1 bound in the initial steps. The color is measured by spectrophotometrically with wavelength of 450nm. The concentration of COL1A1 in samples is then determined by comparing the O.D. of the samples to the standard curve.

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