Human/Canine/Porcine Insulin ELISA kit

NE01I0004 Human/Canine/Porcine elisa insulin kit

Human/Canine/Porcine elisa for insulin kit is suitable for the detection of samples from human species. Insulin can also be called ILPR, Insulin A chain, Insulin B chain, IRDN, INS, IDDM, IDDM1, IDDM2, ILPR, MODY10, insulin, PNDM4.

Principle of the Assay

This elisa kits for insulin measurement is applied to the solid phase sandwich Enzyme Linked Immunosorbent Assay. The microtiter plate has been pre-coated with capture antibody. The antigen to be measured and the biotin-conjugated detection antibody are then added to the microtiter plate wells sequentially to form a capture antibody-antigen-detection antibody complex. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells, and then a TMB substrate solution is added to the wells to incubate. TMB substrate solution turns blue after the oxidation of HRP, and finally turns to yellow immediately after adding the stop solution. Color of TMB substrate positively correlated with total insulin bound in the initial steps. The color is measured by spectrophotometrically with wavelength of 450nm. The concentration of insulin in samples is then determined by comparing the O.D. of the samples to the standard curve.

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