BlueGene Biotech Canine Brain Derived Neurotrophic Factor ELISA kit

E08B0029 Canine Brain Derived Neurotrophic Factor ELISA kit

Canine Brain Derived Neurotrophic Factor BDNF ELISA kit is suitable for the detection of samples from Canine species. Brain Derived Neurotrophic Factor can also be called Neurotrophin, Abrineurin, BDNF.

Specifications of Canine Brain Derived Neurotrophic Factor ELISA kit
Product Information
Cat. No. E08B0029
Product Name Canine Brain Derived Neurotrophic Factor ELISA kit
Species Canine
Product Size 48 Tests / 96 Tests
Concentration 50-1000 pg/ml
Sensitivity 1.0 pg/ml
Principal Competitive ELISA
Sample Volume 100 ul
Sample Type Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time 90 minutes
Platform Microplate Reader
Conjugate HRP
Detection Method Colorimetric
Storage 2-8®C

Kit Components
MATERIALS SPECIFICATION QUANTITY
MICROTITER PLATE 96 wells stripwell
ENZYME CONJUGATE 6.0 mL 1 vial
STANDARD A (0.5mL) 0 pg/mL 1 vial
STANDARD B (0.5mL) 50 pg/mL 1 vial
STANDARD C (0.5mL) 100 pg/mL 1 vial
STANDARD D (0.5mL) 250 pg/mL 1 vial
STANDARD E (0.5mL) 500 pg/mL 1 vial
STANDARD F (0.5mL) 1000 pg/mL 1 vial
SUBSTRATE A 6 mL 1 vial
SUBSTRATE B 6 mL 1 vial
STOP SOLUTION 6 mL 1 vial
WASH SOLUTION (100 x) 10 mL 1 vial
BALANCE SOLUTION 3 mL 1 vial

Principle of the Assay

BDNF ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for BDNF. Standards or samples are then added to the microtiter plate wells and BDNF if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of BDNF present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for BDNF kit are added to each well to “sandwich” the BDNF immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain BDNF and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BDNF concentration in each sample is interpolated from this standard curve.

Quality Control on Canine Brain Derived Neurotrophic Factor Bluegene ELISA kit
Coefficient of Variance Intra Variation% ＜10%
Inter Variation% ＜12%
Recovery 89-103%
Linearity Diluent Ratio Range %
1:02 88-105
1:04 86-105
1:08 83-108
Specificity/Cross-reactivity No significant cross-reactivity or interference between BDNF and analogues was observed.

Citations of Canine Brain Derived Neurotrophic Factor ELISA kit
E08B0029 has been referenced in the below publications:

Effects of Electroacupuncture on Plasma Concentrations of Three Related Neurotrophic Factors in Normal Dogs.

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